Microbiological investigation of soil, water, food and clinical samples; Antimicrobial activity of nanomaterials; Isolation and partial identification of native microorganisms from ecological sites; Biochemical studies; Extraction of marine bacterial pigments and characterization; Studying of bacterial EPS macromolecules; Investigation of bacterial viruses from environmental samples; Bacterial surface Nanostructural protein layers and its applications.

Doctoral research Ph.D., Science and Humanities at Anna University, Chennai (Course Completed)

Dr. P. Prabu, Associate Professor & Head, Centre for Nanoscience & Technology, KSR College of Technology, India.

"Bacterial exopolymeric liganded mesocage biogranules in vermicomposting to chromium bioaugmentation"

Additional research involvements

"Bacterial exopolymeric substances mediated synthesis of silver nanoparticles and its antibacterial efficiency"

"Development of uplift air mediated bubble column bioreator for green bioenergy Microbial Fuel Cells"

"Isolation and Identification of leather degradation bacteria from the toxic leather chemicals polluted environments"

"Development of Algae derived Biofuel - FAME"

Doctoral research Ph.D., Science and Humanities (Bioscience) at Anna University of Technology, Coimbatore (Changed)

Dr. R. Selvakumar, Asst. Professor, PSG Institute of Advanced Studies, India.

"Development of surface layer protein based bioceramics for the removal of radioactive nuclides"

Additional research involvements

"Bacterial exopolymeric substances mediated synthesis of silver nanoparticles and its antibacterial efficiency"

"Development of biosensor based on bacterial surface layer protein for the detection uranium by micro-electro conductivity measurements"

"Isolation and Identification of dye degradation bacteria from the organic toxic chemicals polluted environments"

"Development of self assembled flagellar proteins on graphite sheet electrode for Microbial Fuel Cells"

Master of Science in Biotechnology (2006-2008)

Dr. M. Ramesh, Asst. Professor, Dept. of Biotechnology, Alagappa University, India.

"Optimization of tissue culture conditions for Agrobacterium tumefaciens mediated transformations in Indica Rice variety ADT 43, using Embryogenic calli and intact explants like Embryo, Leaf base”.

Dr. V. P. Venugopalan, SO/G, Head, BBPS, WSCD, IGCAR (DAE), India.

"Synthesis characterization and antibacterial activities of starch stabilized silver nanoparticles"

Dr. S. Jayachandran, Head, Dept. of Biotechnology, Pondicherry University, India.

Dr. T. Subba Rao, SO/F, BBPS, WSCD, IGCAR (DAE), India.

"Biofilm formation and microbial influenced corrosion of the fire water pipeline system"

Bachelor of Science in Microbiology (2002-2005)

Dr. S. Murugan, (right now @) Asst. Professor, Karunya University, India.

"Isolation and identification of pathogenic microorganisms from common house hold cockroache"

"Microbial contamination study of various river water sources in Erode District (Tamil Nadu State), India"

Cumulative Research Profile (January 2009 – June 2013)

List of other Professional Experiences – Industry / Academia / Research Assistant / Marketing

Technical Research Guidance / Co-Supervisor / Mentor / Support to thesis (2012-15)

Computing Skills

Research Skills in Bio Molecular techniques

• Environmental Microbiology

Microbiological investigation of environmental samples. Biochemical characterization of bacterial consortium in biofilm community. Isolation of pure bacterial culture from various environments. Screening and identification of enzymes producing microorganisms. Molecular analysis for genetic level identification of bacteria.

• Recombinant DNA technology

Isolation of Plasmid and Chromosomal DNA. Isolation of RNA from Gram negative bacteria. Partial digestion of chromosomal DNA with restriction enzyme.16S rDNA amplification and cloning in T/A vector. Growth and maintenance of Caenorhabditiselegans. Isolation of genomic DNA and RNA from different stages of C. elegans.

• Biochemical techniques

Protein estimation (Lowry’s and Bradford method).Carbohydrate estimation (DNSA and O-touledene method).Enzyme assays (SGOT, SGPT, ALP, ACP, Amylase and Lipases).Chromatography (Paper, TLC and Gel Permeation).Gel Electrophoresis for protein and nucleic acids

• Molecular genetics techniques

Single colony isolation and checking for genetic markers. Titration of phages and burst size. Induced mutagenesis and isolation of antibiotic resistant and auxotrophic mutants. Enrichment methods for antibiotic resistant and auxotrophic mutants.Transposon mutagenesis of chromosomal and plasmid DNA.Isolation of specialized transducing phage.

• Immunological techniques

Detection of antigen – antibody reactions: agglutination and precipitin tests. Immuno-diffusion and immune-electrophoresis. Immuno-histochemical staining. Blood cell counts – Total RBC, WBC, and differential count of WBC. Immuno diagnostic procedures – ELISA, western blotting.

• Plant Tissue Culture

Callus initiation and maintenance from various explants. Synthetic seed preparation from intact re-generable explants of medicinal plants. Micro propagation of economically and commercially important medicinal plants.

• Plant Molecular Biology

Genomic DNA extraction and purification from representative monocot, dicot and medicinal plants. Isolation of total RNA from plants. Restriction digestion analysis of plant genomic DNA. Agrobacterium mediated transformation of monocot and dicot plants. Isolation and purification of Ti-plasmid DNA. Hygromycin sensitivity assay.

• Plant Biotechnology / Bioinformatics

Isolation of total RNA from plants. Restriction digestion analysis of plant genomic DNA. Sequence alignment; Similarity searching using FASTA and BLAST. Multiple sequence alignment; Phylogenetic analysis. Restriction mapping and Primer design using programs. Drug designing concepts.RNA structure analysis.

• Nano-Biotechnology / Chemistry

Synthesis and characterization of silver nanoparticles by bio-organic method. Nanofirbers: Scaffolds preparations and coatings. Bio components: Protein nanostructures and paracrystalization. Nanoscale biocomponents from bacterial system. Biological specimen preparations and characterization by using ultra-fine instruments. Bioreactor Design.